Anti-DDX27 Polyclonal Antibody is a Rabbit antibody targeting DDX27. Anti-DDX27 Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Chicken,Dog,Cow,Horse,Sheep)

Blank control (black line): HepG2.
Primary Antibody (green line): Rabbit Anti-DDX27 antibody (TMAB-05040)
Dilution: 2 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human DDX27

Human

DDX27

Probable ATP-dependent RNA helicase DDX27

Probable ATP-dependent RNA helicase.