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Anti-DDIT3 Antibody (2G599) (Synonyms: Growth arrest and DNA damage-inducible protein GADD153, GADD153, DNA da...)

Catalog No. TMAC-01128 Copy Product Info
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Anti-DDIT3 Antibody (2G599) is a Rabbit antibody targeting DDIT3. Anti-DDIT3 Antibody (2G599) can be used in FCM,IHC,WB.

Anti-DDIT3 Antibody (2G599)

Copy Product Info
🥰Excellent
Catalog No. TMAC-01128
Synonyms Growth arrest and DNA damage-inducible protein GADD153, GADD153, DNA damage-inducible transcript 3 protein, DDIT-3, DDIT3, CHOP-10, CHOP10, CHOP, CCAAT/enhancer-binding protein homologous protein, C/EBP-homologous protein 10, C/EBP-homologous protein, C/EBP zeta

Anti-DDIT3 Antibody (2G599) is a Rabbit antibody targeting DDIT3. Anti-DDIT3 Antibody (2G599) can be used in FCM,IHC,WB.

Anti-DDIT3 Antibody
(2G599)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2967-10 days7-10 days
100 μL$4987-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-DDIT3 Antibody (2G599) is a Rabbit antibody targeting DDIT3. Anti-DDIT3 Antibody (2G599) can be used in FCM,IHC,WB.
SynonymsGrowth arrest and DNA damage-inducible protein GADD153, GADD153, DNA damage-inducible transcript 3 protein, DDIT-3, DDIT3, CHOP-10, CHOP10, CHOP, CCAAT/enhancer-binding protein homologous protein, C/EBP-homologous protein 10, C/EBP-homologous protein, C/EBP zeta
Ig Type
IgG
Clone
2G599
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of DDIT3 on PC-12 cells lysates using anti-DDIT3 antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.
6. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.
7. Flow cytometric analysis of Hela cells with DDIT3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Application
Recommended Dose
WB: 1:1000; IHC: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundGADD 153 has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. This protein has also been designated C/EBP-homologous protein (CHOP-10). GADD 153 expression is induced by a variety of cellular stresses, inducing nutrient deprivation and metabolic perturbations. GADD 153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD 153 dimers away from "classical" C/EBP binding sites, recognizing instead unique "nonclassical" sites. Thus GADD 153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells, similar to the regulatory function of Id on the activity of Myo D and Myo D-related proteins involved in the development of muscle cells.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 25 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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