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Anti-DDIT3 Antibody (2G599) is a Rabbit antibody targeting DDIT3. Anti-DDIT3 Antibody (2G599) can be used in WB,IHC,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $296 | 7-10 days | |
100 μL | $498 | 7-10 days |
Description | Anti-DDIT3 Antibody (2G599) is a Rabbit antibody targeting DDIT3. Anti-DDIT3 Antibody (2G599) can be used in WB,IHC,FCM. |
Synonyms | Growth arrest and DNA damage-inducible protein GADD153, GADD153, DNA damage-inducible transcript 3 protein, DDIT-3, DDIT3, CHOP-10, CHOP10, CHOP, CCAAT/enhancer-binding protein homologous protein, C/EBP-homologous protein 10, C/EBP-homologous protein, C/EBP zeta |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of DDIT3 on PC-12 cells lysates using anti-DDIT3 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DDIT3 antibody. Counter stained with hematoxylin. 7. Flow cytometric analysis of Hela cells with DDIT3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Application | |
Recommended Dose | WB: 1:1000; IHC: 1:50-200; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | GADD 153 has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. This protein has also been designated C/EBP-homologous protein (CHOP-10). GADD 153 expression is induced by a variety of cellular stresses, inducing nutrient deprivation and metabolic perturbations. GADD 153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD 153 dimers away from "classical" C/EBP binding sites, recognizing instead unique "nonclassical" sites. Thus GADD 153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells, similar to the regulatory function of Id on the activity of Myo D and Myo D-related proteins involved in the development of muscle cells. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 25 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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