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Anti-DBF4B Polyclonal Antibody
Catalog No. TMAB-04994 Copy Product Info
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Anti-DBF4B Polyclonal Antibody is a Rabbit antibody targeting DBF4B. Anti-DBF4B Polyclonal Antibody can be used in WB.
Anti-DBF4B Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-04994
Anti-DBF4B Polyclonal Antibody is a Rabbit antibody targeting DBF4B. Anti-DBF4B Polyclonal Antibody can be used in WB.
Anti-DBF4B Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
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| Description | Anti-DBF4B Polyclonal Antibody is a Rabbit antibody targeting DBF4B. Anti-DBF4B Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Sample: 293T Cell (Human) Lysate at 30 μg Primary: Anti-DBF4B (TMAB-04994) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 18 kD 2. Sample: Hela Cell (Human) Lysate at 30 μg Primary: Anti-DBF4B (TMAB-04994) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 18 kD 3. Sample: U2os Cell (Human) Lysate at 30 μg Primary: Anti-DBF4B (TMAB-04994) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 18 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Note=Predominantly found in soluble fraction but not in the chromatin-bound fraction. |
| Tissue Specificity | Widely expressed. Highly expressed in testis. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | DBF4B is a regulatory subunit for CDC7, a serine-threonine kinase which links cell cycle regulation to genome duplication. The complex CDC7-DBF4B selectively phosphorylates the MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. DBF4B localizes to the nucleus and its expression is cell cycle-regulated. |
| Immunogen | KLH conjugated synthetic peptide: human DBF4B/ASKL1 |
| Antigen Species | Human |
| Gene Name | DBF4B |
| Gene ID | |
| Protein Name | Protein DBF4 homolog B |
| Uniprot ID | |
| Function | Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. |
| Molecular Weight | Theoretical: 18 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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