Anti-DAZAP1 Antibody (9R964) is a Rabbit antibody targeting DAZAP1. Anti-DAZAP1 Antibody (9R964) can be used in WB,FCM,ICC/IF.

IgG

9R964

Human,Mouse,Rat

1. Western blot analysis of Jurkat cell lysate. Using DAZAP1 (TMAB-04990) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 4% Paraformaldehyde-fixed Jurkat (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (DAZAP1) monoclonal Antibody, unconjugated (TMAB-04990) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
3. The Jurkat (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-DAZAP1 antibody (TMAB-04990, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:1000-2000; FCM: 1:50-100; ICC/IF: 1:50-200

Monoclonal

A synthesized peptide: human DAZ associated protein 1

Human

DAZAP1

DAZ-associated protein 1

RNA-binding protein, which may be required during spermatogenesis.