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Anti-DAXX Antibody (9O762)
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Catalog No. TMAC-01118
Alias MGC126246, MGC126245, hDaxx, Fas death domain associated protein, Fas binding protein, ETS1 associated protein 1, EAP1, EAP 1, Death domain associated protein 6, Death associated protein 6, DAP6, DAP 6, CENP-C binding protein, BING2, BING 2
Anti-DAXX Antibody (9O762) is a Rabbit antibody targeting DAXX. Anti-DAXX Antibody (9O762) can be used in WB,ICC,FCM.
Anti-DAXX Antibody (9O762)
Anti-DAXX Antibody (9O762)
😃Good
Catalog No. TMAC-01118Alias MGC126246, MGC126245, hDaxx, Fas death domain associated protein, Fas binding protein, ETS1 associated protein 1, EAP1, EAP 1, Death domain associated protein 6, Death associated protein 6, DAP6, DAP 6, CENP-C binding protein, BING2, BING 2
Anti-DAXX Antibody (9O762) is a Rabbit antibody targeting DAXX. Anti-DAXX Antibody (9O762) can be used in WB,ICC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-DAXX Antibody (9O762) is a Rabbit antibody targeting DAXX. Anti-DAXX Antibody (9O762) can be used in WB,ICC,FCM. |
| Synonyms | MGC126246, MGC126245, hDaxx, Fas death domain associated protein, Fas binding protein, ETS1 associated protein 1, EAP1, EAP 1, Death domain associated protein 6, Death associated protein 6, DAP6, DAP 6, CENP-C binding protein, BING2, BING 2 |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of Daxx on different lysates using anti-Daxx antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: A549, Lane 3: SW480. 2. ICC staining Daxx in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining Daxx in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. Flow cytometric analysis of Hela cells with Daxx antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Activation of the cell surface receptor FAS by FAS ligand leads to the initiation of apoptosis, a process necessary for the regulation of the immune system and tissue homeostasis. FAS-mediated apoptosis appears to involve a number of divergent and overlapping pathways. Daxx appears to be a central component of a FAS-mediated apoptotic pathway involving the activation of Jun N-terminal kinase (JNK). Although Daxx itself does not contain a death domain, it specifically binds to the death domain of FAS. Overexpression of Daxx activates the JNK pathway and enhances FAS-mediated apoptosis. The Daxx apoptotic pathway acts cooperatively with but is distinct from the FAS-mediated pathway that involves interactions between the death domain-containing protein FADD and the cysteine protease FLICE. Unlike the FAS-FADD-FLICE pathway, the Daxx pathway is sensitive to the apoptotic inhibitor protein Bcl-2. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 81 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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