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Anti-DARS Polyclonal Antibody

Catalog No. TMAB-04985 Copy Product Info
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Anti-DARS Polyclonal Antibody is a Rabbit antibody targeting DARS. Anti-DARS Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-DARS Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-04985

Anti-DARS Polyclonal Antibody is a Rabbit antibody targeting DARS. Anti-DARS Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-DARS Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2207-10 days7-10 days
100 μL$3717-10 days7-10 days
200 μL$5297-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-DARS Polyclonal Antibody is a Rabbit antibody targeting DARS. Anti-DARS Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Sample: Liver (Mouse) Lysate at 40 μg
Primary: Anti-DARS (TMAB-04985) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
2. Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DARS) Polyclonal Antibody, Unconjugated (TMAB-04985) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundAspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Cell proliferation inducing protein 40
Antigen Species
Human
Gene Name
DARS1
Gene ID
Protein Name
Aspartate--tRNA ligase, cytoplasmic
Uniprot ID
Function
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
Chemical Properties
Molecular WeightTheoretical: 57 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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