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Anti-DAPK1 Antibody (4N343)
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Catalog No. TMAB-04977
Anti-DAPK1 Antibody (4N343) is a Rabbit antibody targeting DAPK1. Anti-DAPK1 Antibody (4N343) can be used in IHC-P,IHC-Fr,ICC/IF,IF.
Anti-DAPK1 Antibody (4N343)
Anti-DAPK1 Antibody (4N343)
Copy Product Info😃Good
Catalog No. TMAB-04977
Anti-DAPK1 Antibody (4N343) is a Rabbit antibody targeting DAPK1. Anti-DAPK1 Antibody (4N343) can be used in IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $151 | 7-10 days | 7-10 days | |
| 50 μL | $262 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-DAPK1 Antibody (4N343) is a Rabbit antibody targeting DAPK1. Anti-DAPK1 Antibody (4N343) can be used in IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG |
| Clone | 4N343 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DAPK1 Monoclonal Antibody, Unconjugated (TMAB-04977) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DAPK1 Monoclonal Antibody, Unconjugated (TMAB-04977) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DAPK1 Monoclonal Antibody, Unconjugated (TMAB-04977) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DAPK1 Monoclonal Antibody, Unconjugated (TMAB-04977) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DAPK1 Monoclonal Antibody, Unconjugated (TMAB-04977) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DAPK1 Monoclonal Antibody, Unconjugated (TMAB-04977) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DAPK1 Monoclonal Antibody, Unconjugated (TMAB-04977) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Colocalizes with the actin filament system. |
| Tissue Specificity | Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. |
| Immunogen | A synthesized peptide: human DAP kinase 1 |
| Antigen Species | Human |
| Gene Name | DAPK1 |
| Gene ID | |
| Protein Name | Death-associated protein kinase 1 |
| Uniprot ID | |
| Function | Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. |
| Molecular Weight | Theoretical: 160 kDa. Actual: 160 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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