Anti-DALRD3 Polyclonal Antibody is a Rabbit antibody targeting DALRD3. Anti-DALRD3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Horse)

1. Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-DALRD3 Polyclonal Antibody, Unconjugated (TMAB-04972) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-DALRD3 Polyclonal Antibody, Unconjugated (TMAB-04972) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Sample: Liver (Mouse) Lysate at 40 μg
Primary: Anti-DALRD3 (TMAB-04972) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human DALRD3

Human

DALRD3

DALR anticodon-binding domain-containing protein 3

DALRD3 contains a DALR anticodon binding domain. This all alpha helical domain is the anticodon binding domain in Arginyl and glycyl tRNA synthetase. It is known as the DALR domain after characteristic conserved amino acids. There are 5 named isoforms of DALRD3 produced by alternative splicing.