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Anti-Cytokeratin 8 Antibody (3S817)

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Catalog No. TMAY-01277
Alias KO, K8, K2C8, Cytokeratin8, CYK8, CK-8, CK8, CARD2

Anti-Cytokeratin 8 Antibody (3S817) is a Mouse antibody targeting Cytokeratin 8. Anti-Cytokeratin 8 Antibody (3S817) can be used in IHC-P,ICC/IF.

Anti-Cytokeratin 8 Antibody (3S817)

Anti-Cytokeratin 8 Antibody (3S817)

😃Good
Catalog No. TMAY-01277Alias KO, K8, K2C8, Cytokeratin8, CYK8, CK-8, CK8, CARD2
Anti-Cytokeratin 8 Antibody (3S817) is a Mouse antibody targeting Cytokeratin 8. Anti-Cytokeratin 8 Antibody (3S817) can be used in IHC-P,ICC/IF.
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100 μL$198 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Cytokeratin 8 Antibody (3S817) is a Mouse antibody targeting Cytokeratin 8. Anti-Cytokeratin 8 Antibody (3S817) can be used in IHC-P,ICC/IF.
AliasKO, K8, K2C8, Cytokeratin8, CYK8, CK-8, CK8, CARD2
Ig Type
Monoclonal Mouse IgG1
Clone
3S817
Reactivity
Human
Verified Activity
1. Immunochemical staining of human Cytokeratin 8 in human liver with mouse monoclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.

2. Immunochemical staining of human Cytokeratin 8 in human prostate with mouse monoclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.

3. Immunochemical staining of human Cytokeratin 8 in human colon carcinoma with mouse monoclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.

4. Immunochemical staining of human Cytokeratin 8 in human small intestine with mouse monoclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.

5. Immunofluorescence staining of KRT8 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Human KRT8 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
Application
IHC-P,ICC/IF
Recommended Dose
IHC-P: 1:500-1:5000; ICC-IF: 1:20-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the Human Cytokeratin 8. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundKRT8 (Keratin 8) is a Protein Coding gene. 2 alternatively spliced human isoforms have been reported. This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. KRT8 is the major component of the intermediate filament cytoskeleton and is predominantly expressed in simple epithelial tissues. Aberrant expression of KRT8 is associated with multiple tumor progression and metastasis.KRT8 overexpression in renal cancer cell lines promoted cell migration and invasion. In contrast, KRT8 knockdown suppressed ccRCC metastasis both in vitro and in vivo.KRT8 expression may therefore be a biomarker or potential therapeutic target to identify patients with worse survival. Diseases associated with KRT8 include Liver Cirrhosis and Cryptogenic Cirrhosis.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human Cytokeratin 8
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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