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Anti-Cytokeratin 6 Polyclonal Antibody 2
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Catalog No. TMAB-04958
Anti-Cytokeratin 6 Polyclonal Antibody 2 is a Rabbit antibody targeting Cytokeratin 6. Anti-Cytokeratin 6 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Cytokeratin 6 Polyclonal Antibody 2
Anti-Cytokeratin 6 Polyclonal Antibody 2
Copy Product Info😃Good
Catalog No. TMAB-04958
Anti-Cytokeratin 6 Polyclonal Antibody 2 is a Rabbit antibody targeting Cytokeratin 6. Anti-Cytokeratin 6 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Cytokeratin 6 Polyclonal Antibody 2 is a Rabbit antibody targeting Cytokeratin 6. Anti-Cytokeratin 6 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Cytokeratin 6 Polyclonal Antibody, Unconjugated (TMAB-04958) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Cytokeratin 6 Polyclonal Antibody, Unconjugated (TMAB-04958) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 3. Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CK6B Polyclonal Antibody, Unconjugated (TMAB-04958) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 4. Tissue/cell: Human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Cytokeratin 6 Polyclonal Antibody, Unconjugated (TMAB-04958) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 5. Sample: Lane 1: Mouse Skin tissue lysates Lane 2: Mouse Placenta tissue lysates Lane 3: Mouse Lung tissue lysates Primary: Anti-Cytokeratin 6 (TMAB-04958) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Tissue Specificity | Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin(KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq]. |
| Immunogen | KLH conjugated synthetic peptide: human Cytokeratin 6 |
| Antigen Species | Human |
| Gene Name | KRT6A |
| Gene ID | |
| Protein Name | Keratin, type II cytoskeletal 6A |
| Uniprot ID |
| Molecular Weight | Theoretical: 60 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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