Anti-Cytokeratin 6 Antibody (7K957) is a Rabbit antibody targeting Cytokeratin 6. Anti-Cytokeratin 6 Antibody (7K957) can be used in IF, IHC-Fr, IHC-P, WB.

IgG

7K957

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 6 Monoclonal Antibody, Unconjugated (TMAB-04960) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 6 Monoclonal Antibody, Unconjugated (TMAB-04960) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 6 Monoclonal Antibody, Unconjugated (TMAB-04960) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Abdominal skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 6 Monoclonal Antibody, Unconjugated (TMAB-04960) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 6 Monoclonal Antibody, Unconjugated (TMAB-04960) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 6 Monoclonal Antibody, Unconjugated (TMAB-04960) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 6 Monoclonal Antibody, Unconjugated (TMAB-04960) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
8. 25 ug total protein per lane of various lysates (see on figure) probed with Cytokeratin 6 monoclonal antibody, unconjugated (TMAB-04960) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

IF=1:200-1000; IHC-Fr=1:200-1000; IHC-P=1:200-1000; WB=1:1000-2000

Monoclonal

A synthesized peptide: human Cytokeratin 6A

Human

KRT6A

Keratin, type II cytoskeletal 6A