Anti-Cytokeratin 20 Antibody (2X563) is a Mouse antibody targeting Cytokeratin 20. Anti-Cytokeratin 20 Antibody (2X563) can be used in IHC-P,IF,IHC-Fr.

IgG2b,k

2X563

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Cytokeratin 20) Monoclonal Antibody, Unconjugated (TMAB-04949) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Cytokeratin 20) Monoclonal Antibody, Unconjugated (TMAB-04949) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Cytokeratin 20) Monoclonal Antibody, Unconjugated (TMAB-04949) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Cytokeratin 20) Monoclonal Antibody, Unconjugated (TMAB-04949) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Cytokeratin 20) Monoclonal Antibody, Unconjugated (TMAB-04949) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Cytokeratin 20) Monoclonal Antibody, Unconjugated (TMAB-04949) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Cytokeratin 20) Monoclonal Antibody, Unconjugated (TMAB-04949) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Anti-Cytokeratin 20 Monoclonal Antibody, Unconjugated (TMAB-04949) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.
9. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Anti-Cytokeratin 20 Monoclonal Antibody, Unconjugated (TMAB-04949) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.
10. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Anti-Cytokeratin 20 Monoclonal Antibody, Unconjugated (TMAB-04949) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.

IHC-P: 1:100-500; IF: 1:100-500; IHC-Fr: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human CK20

Human

KRT20

Keratin, type I cytoskeletal 20

Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).