Anti-Cytokeratin 1 Polyclonal Antibody is a Rabbit antibody targeting Cytokeratin 1. Anti-Cytokeratin 1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rabbit (predicted:Rat,Dog,Cow,Horse)

1. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Cytokeratin 1 Polyclonal Antibody, Unconjugated (TMAB-04931) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: mouse embryos tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Cytokeratin 1 Polyclonal Antibody, Unconjugated (TMAB-04931) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Blank control (blue line): Rabbit spleen cells (blue).
Primary Antibody (green line): Rabbit Anti-Cytokeratin 1/PE Conjugated antibody (TMAB-04931-PE)
Dilution: 0.2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-PE.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
4. Blank control (blue line): Hela cells (blue).
Primary Antibody (green line): Rabbit Anti-Cytokeratin 1/FITC Conjugated antibody (TMAB-04931-PE)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-PE.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
5. Blank control (blue line): MCF7 cells (blue).
Primary Antibody (green line): Rabbit Anti-Cytokeratin 1/FITC Conjugated antibody (TMAB-04931-FITC)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Cytokeratin 1

Human

KRT1

Keratin, type II cytoskeletal 1

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinty receptor for kininogen-1/HMWK.