Anti-Cytochrome P450 17A1 Polyclonal Antibody is a Rabbit antibody targeting Cytochrome P450 17A1. Anti-Cytochrome P450 17A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Cow,Horse,Rabbit,Sheep)

1. Sample: Testis (Mouse) Lysate at 40 μg
Primary: Anti-Cytochrome P450 17A1 (TMAB-04923) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
2. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytochrome P450 17A1) Polyclonal Antibody, Unconjugated (TMAB-04923) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Sample:
Lane 1: Mouse Adrenal gland tissue lysates
Lane 2: Rat Adrenal gland tissue lysates
Primary: Anti-Cytochrome P450 17A1 (TMAB-04923) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 60 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human P45017A1/Cytochrome P450 17A1

Human

CYP17A1

Steroid 17-alpha-hydroxylase/17, 20 lyase

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.