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Anti-Cytochrome b245 Light Chain Polyclonal Antibody
😃Good
Catalog No. TMAB-00530
Alias Superoxide-generating NADPH oxidase light chain subunit, p22-phox, p22phox, p22 phagocyte B-cytochrome, Neutrophil cytochrome b 22 kDa polypeptide, Flavocytochrome b 558 alpha polypeptide, Cytochrome b558 subunit alpha, Cytochrome b(558) alpha subunit, Cytochrome b(558) alpha chain, Cytochrome b alpha polypeptide, Cytochrome b 245 light chain, Cytochrome b 245 alpha polypeptide, CYBA, CY24A
Anti-Cytochrome b245 Light Chain Polyclonal Antibody is a Rabbit antibody targeting Cytochrome b245 Light Chain. Anti-Cytochrome b245 Light Chain Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P.
Anti-Cytochrome b245 Light Chain Polyclonal Antibody
Anti-Cytochrome b245 Light Chain Polyclonal Antibody
😃Good
Catalog No. TMAB-00530Alias Superoxide-generating NADPH oxidase light chain subunit, p22-phox, p22phox, p22 phagocyte B-cytochrome, Neutrophil cytochrome b 22 kDa polypeptide, Flavocytochrome b 558 alpha polypeptide, Cytochrome b558 subunit alpha, Cytochrome b(558) alpha subunit, Cytochrome b(558) alpha chain, Cytochrome b alpha polypeptide, Cytochrome b 245 light chain, Cytochrome b 245 alpha polypeptide, CYBA, CY24A
Anti-Cytochrome b245 Light Chain Polyclonal Antibody is a Rabbit antibody targeting Cytochrome b245 Light Chain. Anti-Cytochrome b245 Light Chain Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Cytochrome b245 Light Chain Polyclonal Antibody is a Rabbit antibody targeting Cytochrome b245 Light Chain. Anti-Cytochrome b245 Light Chain Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P. |
| Synonyms | Superoxide-generating NADPH oxidase light chain subunit, p22-phox, p22phox, p22 phagocyte B-cytochrome, Neutrophil cytochrome b 22 kDa polypeptide, Flavocytochrome b 558 alpha polypeptide, Cytochrome b558 subunit alpha, Cytochrome b(558) alpha subunit, Cytochrome b(558) alpha chain, Cytochrome b alpha polypeptide, Cytochrome b 245 light chain, Cytochrome b 245 alpha polypeptide, CYBA, CY24A |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat) |
| Verified Activity | Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CYBA Polyclonal Antibody, Unconjugated (TMAB-00530) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.  |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell Membrane |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase is present in phagocytes, neuroepithelial bodies, vascular smooth muscle cells and endothelial cells. It includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane, where they associate with the flavocytochrome cytochrome b558 to form the active enzyme complex. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. |
| Immunogen | KLH conjugated synthetic peptide: human CK18 Cytochrome b245 Light Chain/p22phox |
| Antigen Species | Human |
| Gene Name | CYBA |
| Gene ID | |
| Protein Name | Cytochrome b-245 light chain |
| Uniprot ID | |
| Biology Area | Cellular metabolic process,Oxidative Stress,Cytotoxic Cells,Cytochromes,Oxidative stress |
| Function | Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide. |
| Molecular Weight | Theoretical: 21 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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