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Anti-Cytochrome b245 Light Chain Polyclonal Antibody

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Catalog No. TMAB-00530

Anti-Cytochrome b245 Light Chain Polyclonal Antibody is a Rabbit antibody targeting Cytochrome b245 Light Chain. Anti-Cytochrome b245 Light Chain Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-Cytochrome b245 Light Chain Polyclonal Antibody

Anti-Cytochrome b245 Light Chain Polyclonal Antibody

😃Good
Catalog No. TMAB-00530
Anti-Cytochrome b245 Light Chain Polyclonal Antibody is a Rabbit antibody targeting Cytochrome b245 Light Chain. Anti-Cytochrome b245 Light Chain Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$372 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Cytochrome b245 Light Chain Polyclonal Antibody is a Rabbit antibody targeting Cytochrome b245 Light Chain. Anti-Cytochrome b245 Light Chain Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat)
Verified Activity
Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CYBA Polyclonal Antibody, Unconjugated (TMAB-00530) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
Application
IHC-P,IHC-Fr,IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell Membrane
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundMox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase is present in phagocytes, neuroepithelial bodies, vascular smooth muscle cells and endothelial cells. It includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane, where they associate with the flavocytochrome cytochrome b558 to form the active enzyme complex. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CK18 Cytochrome b245 Light Chain/p22phox
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cellular metabolic process,Oxidative Stress,Cytotoxic Cells,Cytochromes,Oxidative stress
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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