Anti-Cystatin B Antibody (1Y419) is a Rabbit antibody targeting Cystatin B. Anti-Cystatin B Antibody (1Y419) can be used in IF, IHC-Fr, IHC-P, WB.

IgG

1Y419

Human,Rat

1. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cystatin B Monoclonal Antibody, Unconjugated (TMAB-04912) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cystatin B Monoclonal Antibody, Unconjugated (TMAB-04912) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cystatin B Monoclonal Antibody, Unconjugated (TMAB-04912) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Skin Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cystatin B Monoclonal Antibody, Unconjugated (TMAB-04912) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. 25 ug total protein per lane of various lysates (see on figure) probed with Cystatin B monoclonal antibody, unconjugated (TMAB-04912) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000

Monoclonal

A synthesized peptide: human Cystatin B

Human

CSTB

Cystatin-B

Shows moderate inhibition of cathepsin B but is not active against cathepsin C.