Shopping Cart
Remove All
Your shopping cart is currently empty
Anti-CYPA Polyclonal Antibody
Catalog No. TMAB-04907 Copy Product Info
🥰Excellent
Anti-CYPA Polyclonal Antibody is a Rabbit antibody targeting CYPA. Anti-CYPA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-CYPA Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-04907
Anti-CYPA Polyclonal Antibody is a Rabbit antibody targeting CYPA. Anti-CYPA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-CYPA Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
Add to Cart
Add to Quotation
For research use only—not for human use. No sales to individuals. Use as intended only.
Resource Download
Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-CYPA Polyclonal Antibody is a Rabbit antibody targeting CYPA. Anti-CYPA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit) |
| Verified Activity | 1. Sample: Cerebrum (Rat) Lysate at 40 μg Hela (Human) Cell Lysate at 30 μg Primary: Anti-CYPA (TMAB-04907) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 17/18 kD 2. Sample: NIH/3T3 (Mouse) Cell Lysate at 30 μg A431 (Human) Cell Lysate at 30 μg Primary: Anti-CYPA (TMAB-04907) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 18 kD 3. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CYPA Polyclonal Antibody, Unconjugated (TMAB-04907) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 4. Blank control (blue): Hela (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-CYPA antibody (TMAB-04907), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA. 5. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CYPA Polyclonal Antibody, Unconjugated (TMAB-04907) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 6. Sample: Lane 1: Mouse Thymus tissue lysates Lane 2: Mouse Lymph node tissue lysates Lane 3: Mouse Lung tissue lysates Lane 4: Mouse NIH/3T3 cell lysates Lane 5: Rat Thymus tissue lysates Lane 6: Rat Lymph node tissue lysates Lane 7: Rat Lung tissue lysates Lane 8: Human Hela cell lysates Lane 9: Human PANC-1 cell lysates Lane 10: Human Molt-4 cell lysates Lane 11: Human HUVEC cell lysates Lane 12: Human THP-1 cell lysates Primary: Anti-CYPA (TMAB-04907) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kDa Observed band size: 18 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The immunosuppressant cyclosporin A (CsA) forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity (1). Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins (2,3). Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of the CsA and is encoded by a single unique gene conserved from yeast to humans (4,5). CyPA is known for its involvement in T cell differentiation and proliferation and is highly expressed in brain (6). CyPA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication (7,8). Cyclophilin B (CyPB) is a member of the cyclophilin family with specific N- and C-terminal extensions. Unlike CyPA, CyPB has a signal sequence leading to its translocation in the endoplasmic reticulum. CyPB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes (9,10). |
| Immunogen | KLH conjugated synthetic peptide: human Cyclophilin A. |
| Antigen Species | Human |
| Gene Name | PPIA |
| Gene ID | |
| Protein Name | Peptidyl-prolyl cis-trans isomerase A |
| Uniprot ID | |
| Function | PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. |
| Molecular Weight | Theoretical: 18 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Calculator
Preview
Related Tags: buy Anti-CYPA Polyclonal Antibody | purchase Anti-CYPA Polyclonal Antibody | Anti-CYPA Polyclonal Antibody cost | order Anti-CYPA Polyclonal Antibody | Anti-CYPA Polyclonal Antibody molecular weight
Generate Quote
Catalog No.: Cas No.:
Add
| Size | Quantity | Unit Price | Amount | Operation |
|---|
Generate Quote
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2026 TargetMol Chemicals Inc. All Rights Reserved.