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Anti-CYPA Polyclonal Antibody
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Catalog No. TMAB-04907
Anti-CYPA Polyclonal Antibody is a Rabbit antibody targeting CYPA. Anti-CYPA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-CYPA Polyclonal Antibody
Anti-CYPA Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-04907
Anti-CYPA Polyclonal Antibody is a Rabbit antibody targeting CYPA. Anti-CYPA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-CYPA Polyclonal Antibody is a Rabbit antibody targeting CYPA. Anti-CYPA Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit) |
| Verified Activity | 1. Sample: Cerebrum (Rat) Lysate at 40 μg Hela (Human) Cell Lysate at 30 μg Primary: Anti-CYPA (TMAB-04907) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 17/18 kD 2. Sample: NIH/3T3 (Mouse) Cell Lysate at 30 μg A431 (Human) Cell Lysate at 30 μg Primary: Anti-CYPA (TMAB-04907) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 18 kD 3. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CYPA Polyclonal Antibody, Unconjugated (TMAB-04907) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 4. Blank control (blue): Hela (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-CYPA antibody (TMAB-04907), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA. 5. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CYPA Polyclonal Antibody, Unconjugated (TMAB-04907) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 6. Sample: Lane 1: Mouse Thymus tissue lysates Lane 2: Mouse Lymph node tissue lysates Lane 3: Mouse Lung tissue lysates Lane 4: Mouse NIH/3T3 cell lysates Lane 5: Rat Thymus tissue lysates Lane 6: Rat Lymph node tissue lysates Lane 7: Rat Lung tissue lysates Lane 8: Human Hela cell lysates Lane 9: Human PANC-1 cell lysates Lane 10: Human Molt-4 cell lysates Lane 11: Human HUVEC cell lysates Lane 12: Human THP-1 cell lysates Primary: Anti-CYPA (TMAB-04907) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kDa Observed band size: 18 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The immunosuppressant cyclosporin A (CsA) forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity (1). Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins (2,3). Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of the CsA and is encoded by a single unique gene conserved from yeast to humans (4,5). CyPA is known for its involvement in T cell differentiation and proliferation and is highly expressed in brain (6). CyPA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication (7,8). Cyclophilin B (CyPB) is a member of the cyclophilin family with specific N- and C-terminal extensions. Unlike CyPA, CyPB has a signal sequence leading to its translocation in the endoplasmic reticulum. CyPB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes (9,10). |
| Immunogen | KLH conjugated synthetic peptide: human Cyclophilin A. |
| Antigen Species | Human |
| Gene Name | PPIA |
| Gene ID | |
| Protein Name | Peptidyl-prolyl cis-trans isomerase A |
| Uniprot ID | |
| Function | PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. |
| Molecular Weight | Theoretical: 18 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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