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Anti-CYP2R1 Polyclonal Antibody
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Catalog No. TMAB-04891
Anti-CYP2R1 Polyclonal Antibody is a Rabbit antibody targeting CYP2R1. Anti-CYP2R1 Polyclonal Antibody can be used in WB,FCM.
Anti-CYP2R1 Polyclonal Antibody
Anti-CYP2R1 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-04891
Anti-CYP2R1 Polyclonal Antibody is a Rabbit antibody targeting CYP2R1. Anti-CYP2R1 Polyclonal Antibody can be used in WB,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-CYP2R1 Polyclonal Antibody is a Rabbit antibody targeting CYP2R1. Anti-CYP2R1 Polyclonal Antibody can be used in WB,FCM. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Horse,Rabbit) |
| Verified Activity | 1. Sample: A431 (Human) Cell Lysate at 30 μg Primary: Anti-CYP2R1 (TMAB-04891) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD 2. Sample: liver (Human) Cell Lysate at 40 μg Primary: Anti-CYP2R1 (TMAB-04891) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD 3. Sample: A431 (Human) Cell Lysate at 30 μg Primary: Anti-CYP2R1 (TMAB-04891) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 53 kD 4. Blank control: Jurkat. Primary Antibody (green line): Rabbit Anti-CYP2R1 antibody (TMAB-04891) Dilution: 2 μg/Test; Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 0.5 μg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
| Application | |
| Recommended Dose | WB: 1:500-2000; FCM: 2μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
| Tissue Specificity | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | CYP2R1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are mono-oxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor. Defects in CYP2R1 are a cause of 25-hydroxyvitamin D(3) deficiency, also known as pseudovitamin D(3) deficiency rickets due to 25-hydroxylase deficiency. First described in patients who had rickets at a young age despite a history of adequate vitamin D intake. The patients sera had low calcium concentrations, low phosphate concentrations, elevated alkaline phosphatase activity and low levels of 25-hydroxyvitamin D. |
| Immunogen | KLH conjugated synthetic peptide: human CYP2R1 |
| Antigen Species | Human |
| Gene Name | CYP2R1 |
| Gene ID | |
| Protein Name | Vitamin D 25-hydroxylase |
| Uniprot ID |
| Molecular Weight | Theoretical: 55 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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