Anti-CYP2E1 Polyclonal Antibody is a Rabbit antibody targeting CYP2E1. Anti-CYP2E1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Sample: Hela (Human) Cell Lysate at 30 μg
Primary: Anti-CYP2E1 (TMAB-04888) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
2. Sample: Liver (Mouse) Lysate at 40 μg
Primary: Anti-CYP2E1 (TMAB-04888) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
3. Sample: HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-CYP2E1 (TMAB-04888) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
4. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CYP2E1 Polyclonal Antibody, Unconjugated (TMAB-04888) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
5. Blank control: U937.
Primary Antibody (green line): Rabbit Anti-CYP2E1 antibody (TMAB-04888)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CYP2E1

Human

CYP2E1

Cytochrome P450 2E1

Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.