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Anti-CYP2C19 Polyclonal Antibody

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Catalog No. TMAB-04882

Anti-CYP2C19 Polyclonal Antibody is a Rabbit antibody targeting CYP2C19. Anti-CYP2C19 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-CYP2C19 Polyclonal Antibody

Anti-CYP2C19 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-04882
Anti-CYP2C19 Polyclonal Antibody is a Rabbit antibody targeting CYP2C19. Anti-CYP2C19 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3717-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-CYP2C19 Polyclonal Antibody is a Rabbit antibody targeting CYP2C19. Anti-CYP2C19 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CYP2C19) Polyclonal Antibody, Unconjugated (TMAB-04882) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample:
HepG2 (Human) Cell Lysate at 30 μg
A549 (Human) Cell Lysate at 30 μg
Primary: Anti-CYP2C19 (TMAB-04882) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationEndoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
FormulationPreservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
Concentration1mg/ml
Research BackgroundThis gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize many xenobiotics, including the anticonvulsive drug mephenytoin, omeprazole, diazepam and some barbiturates. Polymorphism within this gene is associated with variable ability to metabolize mephenytoin, known as the poor metabolizer and extensive metabolizer phenotypes. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. [provided by RefSeq]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CYP2C19
Antigen Species
Human
Gene Name
CYP2C19
Gene ID
Protein Name
Cytochrome P450 2C19
Uniprot ID
Function
Responsible for the metabolism of a number of therapeutic agents such as the anticonvulsant drug S-mephenytoin, omeprazole, proguanil, certain barbiturates, diazepam, propranolol,
Chemical Properties
Molecular WeightTheoretical: 56 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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