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Anti-CYP27A1 Antibody (5U325)
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Catalog No. TMAC-01060
Alias Vitamin D(3) 25 hydroxylase, Sterol 27 hydroxylase, Sterol 26 hydroxylase mitochondrial, Sterol 26 hydroxylase, Cytochrome P450 subfamily XXVIIA(steroid 27-hydroxylase cerebrotendinous xanthomatosis) polypeptide 1, Cytochrome P450 family 27 subfamily A polypeptide 1, Cytochrome P450 27 mitochondrial, Cytochrome P450 27, Cytochrome P 450C27/25, CYP27, CYP, CTX, CP27A, CP27, Cholestanetriol 26 monooxygenase, 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase, 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase
Anti-CYP27A1 Antibody (5U325) is a Rabbit antibody targeting CYP27A1. Anti-CYP27A1 Antibody (5U325) can be used in WB,ICC,IHC,FCM.
Anti-CYP27A1 Antibody (5U325)
Anti-CYP27A1 Antibody (5U325)
😃Good
Catalog No. TMAC-01060Alias Vitamin D(3) 25 hydroxylase, Sterol 27 hydroxylase, Sterol 26 hydroxylase mitochondrial, Sterol 26 hydroxylase, Cytochrome P450 subfamily XXVIIA(steroid 27-hydroxylase cerebrotendinous xanthomatosis) polypeptide 1, Cytochrome P450 family 27 subfamily A polypeptide 1, Cytochrome P450 27 mitochondrial, Cytochrome P450 27, Cytochrome P 450C27/25, CYP27, CYP, CTX, CP27A, CP27, Cholestanetriol 26 monooxygenase, 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase, 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase
Anti-CYP27A1 Antibody (5U325) is a Rabbit antibody targeting CYP27A1. Anti-CYP27A1 Antibody (5U325) can be used in WB,ICC,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-CYP27A1 Antibody (5U325) is a Rabbit antibody targeting CYP27A1. Anti-CYP27A1 Antibody (5U325) can be used in WB,ICC,IHC,FCM. |
| Synonyms | Vitamin D(3) 25 hydroxylase, Sterol 27 hydroxylase, Sterol 26 hydroxylase mitochondrial, Sterol 26 hydroxylase, Cytochrome P450 subfamily XXVIIA(steroid 27-hydroxylase cerebrotendinous xanthomatosis) polypeptide 1, Cytochrome P450 family 27 subfamily A polypeptide 1, Cytochrome P450 27 mitochondrial, Cytochrome P450 27, Cytochrome P 450C27/25, CYP27, CYP, CTX, CP27A, CP27, Cholestanetriol 26 monooxygenase, 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase, 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of CYP27A1 on different tissue lysates using anti-CYP27A1 antibody at 1/500 dilution.Positive control: Lane 1: Rat heart, Lane 2: Human liver, Lane 2: Human kidney. 2. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CYP27A1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CYP27A1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-CYP27A1 antibody. Counter stained with hematoxylin. 5. ICC staining CYP27A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining CYP27A1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of HepG2 cells with CYP27A1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. 8. All lanes: CYP27A1 Rabbit mAb TMAC-01060 at 1/1000 dilution, Lane 1: Mouse liver lysates, Lane 2: Mouse kidney lysates, Lane 3: Rat liver lysates Lysates at 20 μg per lane. Secondary All lanes: Goat Anti-Rabbit IgG (HRP) at 1/10000 dilution, Predicted band size: 60 kDa, Observed band size: 55-60 kDa, Exposure time: 2 seconds. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endo-genous and xenobiotic compounds. P450 enzymes can be classified, based on their sequence similarities, into distinct subfamilies, which include CYP1A and CYP2A. Other P450 family members include CYP19, also designated aromatase (P450arom), which catalyzes the conversion of C19 steroids to estrogens in various tissues, including placenta, gonads, adipose tissue, skin and brain. CYP19 expression is controlled by hormonally regulated promoters in different tissues and increased aromatase activity is associated with familial gynecomastia. Also, a polymorphic allele of CYP19 (repeat (TTTA)12) is present in a majority of breast cancer patients. P450 cholesterol 7a-hydroxylase, CYP7A1, is the rate limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D3 25-hydroxylation and is localized to the mitochondria in kidney and liver. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: human CYP27A1 300-500aa |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 60 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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