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Anti-CYP1A1 Polyclonal Antibody
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Catalog No. TMAB-00522
Anti-CYP1A1 Polyclonal Antibody is a Rabbit antibody targeting CYP1A1. Anti-CYP1A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CYP1A1 Polyclonal Antibody
Anti-CYP1A1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00522
Anti-CYP1A1 Polyclonal Antibody is a Rabbit antibody targeting CYP1A1. Anti-CYP1A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CYP1A1 Polyclonal Antibody is a Rabbit antibody targeting CYP1A1. Anti-CYP1A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Pig) |
| Verified Activity | 1. Sample: K562 Cell (Human) Lysate at 30 μg Primary: Anti-CYP1A1 (TMAB-00522) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa 2. Sample: Lung (Mouse) Lysate at 40 μg Primary: Anti-CYP1A1 (TMAB-00522) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa 3. Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CYP1A1) Polyclonal Antibody, Unconjugated (TMAB-00522) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Endoplasmic reticulum |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human CYP1A1 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Drug metabolism,Cancer,Cytochromes,Hypoxia,Lipases,Drug metabolism,Cytochromes,Response to hypoxia,Cellular metabolic process |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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