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Anti-CYP17A1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00521
Alias Steroid 17-alpha-monooxygenase, Steroid 17-alpha-hydroxylase/17,20 lyase, S17AH, Cytochrome P450-C17 (Cytochrome P450c17), Cytochrome P450 17A1, CYPXVII, CYP17A1, CYP17, 20 lyase, 17-alpha-hydroxyprogesterone aldolase

Anti-CYP17A1 Polyclonal Antibody is a Rabbit antibody targeting CYP17A1. Anti-CYP17A1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-CYP17A1 Polyclonal Antibody

Anti-CYP17A1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00521Alias Steroid 17-alpha-monooxygenase, Steroid 17-alpha-hydroxylase/17,20 lyase, S17AH, Cytochrome P450-C17 (Cytochrome P450c17), Cytochrome P450 17A1, CYPXVII, CYP17A1, CYP17, 20 lyase, 17-alpha-hydroxyprogesterone aldolase
Anti-CYP17A1 Polyclonal Antibody is a Rabbit antibody targeting CYP17A1. Anti-CYP17A1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3727-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-CYP17A1 Polyclonal Antibody is a Rabbit antibody targeting CYP17A1. Anti-CYP17A1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
AliasSteroid 17-alpha-monooxygenase, Steroid 17-alpha-hydroxylase/17,20 lyase, S17AH, Cytochrome P450-C17 (Cytochrome P450c17), Cytochrome P450 17A1, CYPXVII, CYP17A1, CYP17, 20 lyase, 17-alpha-hydroxyprogesterone aldolase
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Sheep)
Verified Activity
1. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CYP17 Polyclonal Antibody, Unconjugated (TMAB-00521) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CYP17) Polyclonal Antibody, Unconjugated (TMAB-00521) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CYP17) Polyclonal Antibody, Unconjugated (TMAB-00521) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
Application
IHC-P,IHC-Fr,IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundCytochrome P450 17A1 (CYP17A1) belongs to the cytochrome P450 family; it plays a role in the conversion of pregnenolone and progesterone into their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. CYP17A1 also catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. CYP17A1 is involved in sexual development during fetal life and at puberty. Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5). AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CYP17
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Hormone biosynthesis,Metabolism of lipids and lipoproteins,Hormones,Cytochromes,Hormone biosynthesis,Lipases,Lipid metabolism,Cytochromes,Cancer,Hormones,Lipid metabolism
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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