Anti-CYP17A1 Polyclonal Antibody is a Rabbit antibody targeting CYP17A1. Anti-CYP17A1 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P.

IgG

Mouse (predicted:Human,Rat,Dog,Pig,Cow,Horse,Sheep)

1. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CYP17 Polyclonal Antibody, Unconjugated (TMAB-00521) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CYP17) Polyclonal Antibody, Unconjugated (TMAB-00521) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CYP17) Polyclonal Antibody, Unconjugated (TMAB-00521) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CYP17

Human

CYP17A1

Steroid 17-alpha-hydroxylase/17, 20 lyase

Hormone biosynthesis,Metabolism of lipids and lipoproteins,Hormones,Cytochromes,Hormone biosynthesis,Lipases,Lipid metabolism,Cytochromes,Cancer,Hormones,Lipid metabolism

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.