Anti-CYP11A1 Antibody (5F467) is a Mouse antibody targeting CYP11A1. Anti-CYP11A1 Antibody (5F467) can be used in WB, IHC-P, IHC-Fr, ICC/IF, IF.

IgG1, Kappa

5F467

Human

1. Cell line: HepG2
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-04864
2. Tissue: Human placenta
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: sP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-04864
3. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: room temperature 2 h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: JAR, HepG2
Protein loading quantity: 20 μg
Exposure time: 3 s
Predicted MW: 52 kDa
Observed MW: 52 kDa

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200

Monoclonal

KLH conjugated synthetic peptide: human CYP11A1

Human

CYP11A1

Cholesterol side-chain cleavage enzyme, mitochondrial

Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.