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Anti-Cyclophilin B Antibody (7Z10), Loading Control
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Catalog No. TMAB-04855
Anti-Cyclophilin B Antibody (7Z10), Loading Control is a Rabbit antibody targeting Cyclophilin B. Anti-Cyclophilin B Antibody (7Z10), Loading Control can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Cyclophilin B Antibody (7Z10), Loading Control
Anti-Cyclophilin B Antibody (7Z10), Loading Control
Copy Product Info😃Good
Catalog No. TMAB-04855
Anti-Cyclophilin B Antibody (7Z10), Loading Control is a Rabbit antibody targeting Cyclophilin B. Anti-Cyclophilin B Antibody (7Z10), Loading Control can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Cyclophilin B Antibody (7Z10), Loading Control is a Rabbit antibody targeting Cyclophilin B. Anti-Cyclophilin B Antibody (7Z10), Loading Control can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 7Z10 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: Lane 1: Placenta (Rat) Lysate at 40 μg Lane 2: Liver (Mouse) Lysate at 40 μg Lane 3: Kidney (Mouse) Lysate at 40 μg Lane 4: Cerebrum (Rat) Lysate at 40 μg Lane 5: Liver (Rat) Lysate at 40 μg Lane 6: Kidney (Rat) Lysate at 40 μg Lane 7: Hela (Human) Cell Lysate at 30 μg Lane 8: A431 (Human) Cell Lysate at 30 μg Lane 9: HepG2 (Human) Cell Lysate at 30 μg Lane 10: Huvec (Human) Cell Lysate at 30 μg Lane 11: U937 (Human) Cell Lysate at 30 μg Primary: Anti-Cyclophilin B (TMAB-04855) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kD Observed band size: 18 kD 2. Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cyclophilin b (Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-04855) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cyclophilin b (Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-04855) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The cyclophilins are a conserved class of proteins that bind the immunosuppressive drug cyclosporin A (CsA) with high affinity. CsA blocks helper T-cell activation at a step between T-cell receptor stimulation and the transcriptional activation of cytokine genes. Cyclophilins from many species possess peptidyl-prolyl cis-trans isomerase (PPIase) activity that is blocked by CsA and therefore may be relevant in CsA-mediated immunosuppression. |
| Immunogen | Recombinant Protein: human Cyclophilin B |
| Antigen Species | Human |
| Gene Name | PPIB |
| Gene ID | |
| Protein Name | Peptidyl-prolyl cis-trans isomerase B |
| Uniprot ID | |
| Function | PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. |
| Molecular Weight | Theoretical: 20 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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