Anti-Cyclophilin B Antibody (6Q861), Loading Control is a Mouse antibody targeting Cyclophilin B. Anti-Cyclophilin B Antibody (6Q861), Loading Control can be used in WB,IHC-P,IHC-Fr,IF.

IgG

6Q861

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Cyclophilin b (Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-04853) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Cyclophilin b (Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-04853) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Cyclophilin b (Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-04853) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Cyclophilin b (Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-04853) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin b (Loading Control) Monoclonal Antibody, Unconjugated (TMAB-04853) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Rat Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin b (Loading Control) Monoclonal Antibody, Unconjugated (TMAB-04853) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin b (Loading Control) Monoclonal Antibody, Unconjugated (TMAB-04853) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin b (Loading Control) Monoclonal Antibody, Unconjugated (TMAB-04853) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin b (Loading Control) Monoclonal Antibody, Unconjugated (TMAB-04853) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin b (Loading Control) Monoclonal Antibody, Unconjugated (TMAB-04853) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin b (Loading Control) Monoclonal Antibody, Unconjugated (TMAB-04853) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin b (Loading Control) Monoclonal Antibody, Unconjugated (TMAB-04853) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin b (Loading Control) Monoclonal Antibody, Unconjugated (TMAB-04853) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin b (Loading Control) Monoclonal Antibody, Unconjugated (TMAB-04853) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
15. Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclophilin b (Loading Control) Monoclonal Antibody, Unconjugated (TMAB-04853) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
16. 25 μg total protein per lane of various lysates (see on figure) probed with Cyclophilin B monoclonal antibody, unconjugated (TMAB-04853) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human Cyclophilin B

Human

PPIB

Peptidyl-prolyl cis-trans isomerase B

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.