Anti-Cyclooxygenase 1 Polyclonal Antibody 3 is a Rabbit antibody targeting Cyclooxygenase 1. Anti-Cyclooxygenase 1 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Cow,Sheep)

1. Sample:
NIH/3T3 (Mouse) Cell Lysate at 30 μg
A431 (Human) Cell Lysate at 30 μg
Hela (Human) Cell Lysate at 30 μg
Huvec (Human) Cell Lysate at 30 μg
Primary: Anti-Cyclooxygenase 1 (TMAB-04847) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD
2. Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-COX-1 Polyclonal Antibody, Unconjugated (TMAB-04847) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Cyclooxygenase 1

Human

PTGS1

Prostaglandin G/H synthase 1

May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.