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Anti-Cyclin M2 Polyclonal Antibody
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Catalog No. TMAB-04845
Anti-Cyclin M2 Polyclonal Antibody is a Rabbit antibody targeting Cyclin M2. Anti-Cyclin M2 Polyclonal Antibody can be used in WB,FCM.
Anti-Cyclin M2 Polyclonal Antibody
Anti-Cyclin M2 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-04845
Anti-Cyclin M2 Polyclonal Antibody is a Rabbit antibody targeting Cyclin M2. Anti-Cyclin M2 Polyclonal Antibody can be used in WB,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
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| Description | Anti-Cyclin M2 Polyclonal Antibody is a Rabbit antibody targeting Cyclin M2. Anti-Cyclin M2 Polyclonal Antibody can be used in WB,FCM. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit) |
| Verified Activity | 1. Sample: Brain (Mouse) Lysate at 40 μg Primary: Anti-Cyclin M2 (TMAB-04845) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution Predicted band size: 96 kD Observed band size: 96 kD 2. Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-Cyclin M2 antibody (TMAB-04845) Dilution: 1 μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE Dilution: 1 μg /test. Protocol The cells then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
| Application | |
| Recommended Dose | WB: 1:500-2000; FCM: 3μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. |
| Tissue Specificity | Widely expressed. Expressed at higher level in brain, kidney and placenta, while it is weakly expressed in skeletal muscle. In the kidney, it is expressed in the distal convoluted tubule and the thick ascending limb of Henle loop. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Cyclin M2, also known as CNNM2 or ACDP2 (ancient conserved domain-containing protein 2), is an 875 amino acid multi-pass membrane protein that contains two CBS domains and belongs to the ACDP family. Expressed in a variety of tissues with highest expression in placenta, brain and kidney, cyclin M2 functions as a divalent metal cation transporter that mediates the transport of several different metal cations, including Mg2+, Co2+ and Fe2+. Cyclin M2 exists as multiple alternatively spliced isoforms and, contrary to its name, exhibits no cyclin-like function in vivo. The gene encoding cyclin M2 maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman’s syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria. |
| Immunogen | KLH conjugated synthetic peptide: human Cyclin M2/CNNM2 |
| Antigen Species | Human |
| Gene Name | CNNM2 |
| Gene ID | |
| Protein Name | Metal transporter CNNM2 |
| Uniprot ID | |
| Function | Divalent metal cation transporter. Mediates transport of divalent metal cations in an order of Mg(2+) > Co(2+) > Mn(2+) > Sr(2+) > Ba(2+) > Cu(2+) > Fe(2+ |
| Molecular Weight | Theoretical: 96 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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