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Anti-Cyclin H Antibody (8F15)
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Catalog No. TMAC-01051
Alias p37, p36, p34, MO15-associated protein, MO15 associated protein, Cyclin-H, CyclinH, Cyclin H, cyclin dependent kinase activating kinase complex subunit, Cyclin dependent kinase activating kinase, CDK activating kinase complex subunit, CDK activating kinase, CCNH_HUMAN, ccnh, CAK complex subunit, CAK, AW538719, AV102684, AI661354, 6330408H09Rik
Anti-Cyclin H Antibody (8F15) is a Rabbit antibody targeting Cyclin H. Anti-Cyclin H Antibody (8F15) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-Cyclin H Antibody (8F15)
Anti-Cyclin H Antibody (8F15)
😃Good
Catalog No. TMAC-01051Alias p37, p36, p34, MO15-associated protein, MO15 associated protein, Cyclin-H, CyclinH, Cyclin H, cyclin dependent kinase activating kinase complex subunit, Cyclin dependent kinase activating kinase, CDK activating kinase complex subunit, CDK activating kinase, CCNH_HUMAN, ccnh, CAK complex subunit, CAK, AW538719, AV102684, AI661354, 6330408H09Rik
Anti-Cyclin H Antibody (8F15) is a Rabbit antibody targeting Cyclin H. Anti-Cyclin H Antibody (8F15) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-Cyclin H Antibody (8F15) is a Rabbit antibody targeting Cyclin H. Anti-Cyclin H Antibody (8F15) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | p37, p36, p34, MO15-associated protein, MO15 associated protein, Cyclin-H, CyclinH, Cyclin H, cyclin dependent kinase activating kinase complex subunit, Cyclin dependent kinase activating kinase, CDK activating kinase complex subunit, CDK activating kinase, CCNH_HUMAN, ccnh, CAK complex subunit, CAK, AW538719, AV102684, AI661354, 6330408H09Rik |
| Ig Type | IgG |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Western blot analysis of Cyclin H on K562 cells lysates using anti-Cyclin H antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cyclin H antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cyclin H antibody. Counter stained with hematoxylin. 4. ICC staining Cyclin H in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Cyclin H in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Cyclin H in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Hela cells with Cyclin H antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Progression through the cell cycle requires activation of a series of enzymes designated cyclin dependent kinases (Cdks). The monomeric catalytic subunit, Cdk2, a critical enzyme for initiation of cell cycle progression, is completely inactive. Partial activation is achieved by the binding of regulatory cyclins such as cyclin D1, while full activation requires, in addition, phosphorylation at Thr-160. The enzyme responsible for phosphorylation of Thr-160 in Cdk2 and also Thr-161 in Cdc2 p34, designated Cdk-activating kinase (CAK), has been partially purified and shown to be comprised of a catalytic subunit and a regulatory subunit. The catalytic subunit, designated Cdk7, has been identified as the mammalian homolog of MO15, a protein kinase demonstrated earlier in starfish and Xenopus. The regulatory subunit is a novel cyclin (cyclin H) and is required for activation of Cdk7. Like other Cdks, Cdk7 contains a conserved threonine required for full activity; mutation of this residue severely reduces CAK activity. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 38 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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