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Anti-Cyclin E2 Antibody (2N277)
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Catalog No. TMAC-01049
Alias G1/S specific cyclin E2, CyclinE2, CYCE2, CYCE 2, CYC E2, CCNE2 protein, CCNE2, CCNE 2, CCN E2
Anti-Cyclin E2 Antibody (2N277) is a Rabbit antibody targeting Cyclin E2. Anti-Cyclin E2 Antibody (2N277) can be used in WB,ICC/IF,IHC,FCM.
Anti-Cyclin E2 Antibody (2N277)
Anti-Cyclin E2 Antibody (2N277)
😃Good
Catalog No. TMAC-01049Alias G1/S specific cyclin E2, CyclinE2, CYCE2, CYCE 2, CYC E2, CCNE2 protein, CCNE2, CCNE 2, CCN E2
Anti-Cyclin E2 Antibody (2N277) is a Rabbit antibody targeting Cyclin E2. Anti-Cyclin E2 Antibody (2N277) can be used in WB,ICC/IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-Cyclin E2 Antibody (2N277) is a Rabbit antibody targeting Cyclin E2. Anti-Cyclin E2 Antibody (2N277) can be used in WB,ICC/IF,IHC,FCM. |
| Synonyms | G1/S specific cyclin E2, CyclinE2, CYCE2, CYCE 2, CYC E2, CCNE2 protein, CCNE2, CCNE 2, CCN E2 |
| Ig Type | IgG |
| Clone | 2N277 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cyclin E2 antibody. Counter stained with hematoxylin. 2. ICC staining Cyclin E2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining Cyclin E2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Cyclin E2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of Hela cells with Cyclin E2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. Cyclin E2 is a cyclin E-related protein that specifically interacts with Cdk2 and Cdk3 and with p27 and p21. Cyclin E2 expression peaks at the G1/S phase transition of the cell cycle, in parallel with cyclin E. Whereas cyclin E1 is expressed in most proliferating normal and tumor cells, cyclin E2 levels are low or undetectable in nontransformed cells, and are elevated in tumor-derived cells. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 47 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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