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Anti-Cyclin E1 Antibody (1V718)

Catalog No. TMAB-04835 Copy Product Info
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Anti-Cyclin E1 Antibody (1V718) is a Rabbit antibody targeting Cyclin E1. Anti-Cyclin E1 Antibody (1V718) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-Cyclin E1 Antibody (1V718)

Copy Product Info
🥰Excellent
Catalog No. TMAB-04835

Anti-Cyclin E1 Antibody (1V718) is a Rabbit antibody targeting Cyclin E1. Anti-Cyclin E1 Antibody (1V718) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-Cyclin E1 Antibody
(1V718)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2627-10 days7-10 days
100 μL$4737-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Cyclin E1 Antibody (1V718) is a Rabbit antibody targeting Cyclin E1. Anti-Cyclin E1 Antibody (1V718) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG
Clone
1V718
Reactivity
Human
Verified Activity
1. 25 μg total protein per lane of various lysates (see on figure) probed with Cyclin E1 monoclonal antibody, unconjugated (TMAB-04835) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cyclin E1 Monoclonal Antibody, Unconjugated (TMAB-04835) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cyclin E1 Monoclonal Antibody, Unconjugated (TMAB-04835) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cyclin E1 Monoclonal Antibody, Unconjugated (TMAB-04835) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cyclin E1 Monoclonal Antibody, Unconjugated (TMAB-04835) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cyclin E1 Monoclonal Antibody, Unconjugated (TMAB-04835) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
Tissue SpecificityHighly expressed in testis and placenta. Low levels in bronchial epithelial cells.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundCyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Antigen Details
Immunogen
A synthesized peptide: human Cyclin E1
Antigen Species
Human
Gene Name
CCNE1
Gene ID
Protein Name
G1/S-specific cyclin-E1
Uniprot ID
Function
Essential for the control of the cell cycle at the G1/S (start) transition.
Chemical Properties
Molecular WeightTheoretical: 45 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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