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Anti-Cyclin D3 Antibody (5L448)
😃Good
Catalog No. TMAC-01043
Alias G1/S-specific cyclin-D3, G1/S specific cyclin D3, G1 S specific cyclin D3, D3 type cyclin, CyclinD3, CCND3_HUMAN, Ccnd3, CCND 3
Anti-Cyclin D3 Antibody (5L448) is a Rabbit antibody targeting Cyclin D3. Anti-Cyclin D3 Antibody (5L448) can be used in WB,ICC/IF,FCM.
Anti-Cyclin D3 Antibody (5L448)
Anti-Cyclin D3 Antibody (5L448)
😃Good
Catalog No. TMAC-01043Alias G1/S-specific cyclin-D3, G1/S specific cyclin D3, G1 S specific cyclin D3, D3 type cyclin, CyclinD3, CCND3_HUMAN, Ccnd3, CCND 3
Anti-Cyclin D3 Antibody (5L448) is a Rabbit antibody targeting Cyclin D3. Anti-Cyclin D3 Antibody (5L448) can be used in WB,ICC/IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-Cyclin D3 Antibody (5L448) is a Rabbit antibody targeting Cyclin D3. Anti-Cyclin D3 Antibody (5L448) can be used in WB,ICC/IF,FCM. |
| Synonyms | G1/S-specific cyclin-D3, G1/S specific cyclin D3, G1 S specific cyclin D3, D3 type cyclin, CyclinD3, CCND3_HUMAN, Ccnd3, CCND 3 |
| Ig Type | IgG |
| Clone | 5L448 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Western blot analysis of Cyclin D3 on different lysates using anti-Cyclin D3 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat, Lane 2: Hela. 2. ICC staining Cyclin D3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining Cyclin D3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Cyclin D3 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of Hela cells with Cyclin D3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000; ICC/IF: 1:50-200; FCM: 1:10-50 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The proliferation of eukaryotic cells is controlled at specific points in the cell cycle, particularly at the G1 to S and the G2 to M transitions. It is well established that the Cdc2 p34-cyclin B protein kinase plays a critical role in the G2 to M transition while cyclin A associates with Cdk2 p33 and functions in S phase. Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl-1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 32 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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