Anti-Cyclin D2 Polyclonal Antibody is a Rabbit antibody targeting Cyclin D2. Anti-Cyclin D2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Rat (predicted:Mouse)

1. Tissue/cell: human flat moss tinea tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Cyclin D2 Polyclonal Antibody, Unconjugated (TMAB-04830) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Cyclin D2 Polyclonal Antibody, Unconjugated (TMAB-04830) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Tissue/cell: rat mammary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Cyclin D2 Polyclonal Antibody, Unconjugated (TMAB-04830) 1: 200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated used at 1: 200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml, blue) was used to stain the cell nuclei
4. The figure annotation:
The blue histogram is unstained cells.
The Wathet Blue histogram is cells stained with secondary antibody alone.
The Orange histogram is cells stained with rabbit IgG isotype control antibody plus secondary antibody.
The green histogram is cells stained with Rabbit Anti-Cyclin D2 antibody (TMAB-04830) plus secondary antibody.
Concebtration: 5μg/10^6 cells.
Positive control: MCF-7 cells.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 5μg/Test

Polyclonal

KLH conjugated synthetic peptide: the middle of human Cyclin D2

Human

CCND2

G1/S-specific cyclin-D2

Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.