Anti-Cyclin B3 Polyclonal Antibody is a Rabbit antibody targeting Cyclin B3. Anti-Cyclin B3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Sheep)

1. Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Cyclin B3 Polyclonal Antibody, Unconjugated (TMAB-04827) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Cyclin B3 antibody (TMAB-04827) Dilution: 1 μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE Dilution: 3 μg /test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 3μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Cyclin B3

Human

CCNB3

G2/mitotic-specific cyclin-B3

Cyclins are positive regulatory subunits of thecyclin-dependent kinases (CDKs), and thereby play an essential rolein the control of the cell cycle, notably via their destructionduring cell division. Its tissue specificity suggest that it may berequired during early meiotic prophase I.