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Anti-Cyclin B1 Polyclonal Antibody
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Catalog No. TMAB-04823
Anti-Cyclin B1 Polyclonal Antibody is a Rabbit antibody targeting Cyclin B1. Anti-Cyclin B1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Cyclin B1 Polyclonal Antibody
Anti-Cyclin B1 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-04823
Anti-Cyclin B1 Polyclonal Antibody is a Rabbit antibody targeting Cyclin B1. Anti-Cyclin B1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-Cyclin B1 Polyclonal Antibody is a Rabbit antibody targeting Cyclin B1. Anti-Cyclin B1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Cow,Rabbit,Sheep) |
| Verified Activity | 1. Sample: Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 μg Lane 2: Hela (Human) Cell Lysate at 30 μg Lane 3: Jurkat (Human) Cell Lysate at 30 μg Lane 4: U2os (Human) Cell Lysate at 30 μg Lane 5: K562 (Human) Cell Lysate at 30 μg Primary: Anti-Cyclin B1 (TMAB-04823) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55-60 kD Observed band size: 60 kD 2. Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cyclin B1) Polyclonal Antibody, Unconjugated (TMAB-04823) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 3. Sample: Jurkat Cell Lysate at 40 μg Primary: Anti-Cyclin B1 (TMAB-04823) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 45 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human Cyclin B1 |
| Antigen Species | Human |
| Gene Name | CCNB1 |
| Gene ID | |
| Protein Name | G2/mitotic-specific cyclin-B1 |
| Uniprot ID | |
| Function | Essential for the control of the cell cycle at the G2/M (mitosis) transition. |
| Molecular Weight | Theoretical: 48 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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