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Anti-Cyclin B1 Antibody (5Q476)
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Catalog No. TMAB-04824
Anti-Cyclin B1 Antibody (5Q476) is a Rabbit antibody targeting Cyclin B1. Anti-Cyclin B1 Antibody (5Q476) can be used in WB,ICC/IF,FCM.
Anti-Cyclin B1 Antibody (5Q476)
Anti-Cyclin B1 Antibody (5Q476)
Copy Product Info😃Good
Catalog No. TMAB-04824
Anti-Cyclin B1 Antibody (5Q476) is a Rabbit antibody targeting Cyclin B1. Anti-Cyclin B1 Antibody (5Q476) can be used in WB,ICC/IF,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-Cyclin B1 Antibody (5Q476) is a Rabbit antibody targeting Cyclin B1. Anti-Cyclin B1 Antibody (5Q476) can be used in WB,ICC/IF,FCM. |
| Ig Type | IgG |
| Clone | 5Q476 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Cyclin B1) monoclonal Antibody, unconjugated (TMAB-04824) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, FITC) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control. 2. 25 ug total protein per lane of various lysates (see on figure) probed with Cyclin B1 monoclonal antibody, unconjugated (TMAB-04824) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1μg/Test |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]. |
| Immunogen | Recombinant Protein: human Cyclin B1 protein（1-150aa） |
| Antigen Species | Human |
| Gene Name | CCNB1 |
| Gene ID | |
| Protein Name | G2/mitotic-specific cyclin-B1 |
| Uniprot ID | |
| Function | Essential for the control of the cell cycle at the G2/M (mitosis) transition. |
| Molecular Weight | Theoretical: 48 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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