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Anti-Cyclin B1 Antibody (5Q476)

Catalog No. TMAB-04824 Copy Product Info
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Anti-Cyclin B1 Antibody (5Q476) is a Rabbit antibody targeting Cyclin B1. Anti-Cyclin B1 Antibody (5Q476) can be used in WB,ICC/IF,FCM.

Anti-Cyclin B1 Antibody (5Q476)

Copy Product Info
🥰Excellent
Catalog No. TMAB-04824

Anti-Cyclin B1 Antibody (5Q476) is a Rabbit antibody targeting Cyclin B1. Anti-Cyclin B1 Antibody (5Q476) can be used in WB,ICC/IF,FCM.

Anti-Cyclin B1 Antibody
(5Q476)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2637-10 days7-10 days
100 μL$4717-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Cyclin B1 Antibody (5Q476) is a Rabbit antibody targeting Cyclin B1. Anti-Cyclin B1 Antibody (5Q476) can be used in WB,ICC/IF,FCM.
Ig Type
IgG
Clone
5Q476
Reactivity
Human,Mouse
Verified Activity
1. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Cyclin B1) monoclonal Antibody, unconjugated (TMAB-04824) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, FITC) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. 25 ug total protein per lane of various lysates (see on figure) probed with Cyclin B1 monoclonal antibody, unconjugated (TMAB-04824) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1μg/Test
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
Recombinant Protein: human Cyclin B1 protein(1-150aa)
Antigen Species
Human
Gene Name
CCNB1
Gene ID
Protein Name
G2/mitotic-specific cyclin-B1
Uniprot ID
Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Chemical Properties
Molecular WeightTheoretical: 48 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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