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Anti-CYBB Polyclonal Antibody

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Catalog No. TMAB-00514

Anti-CYBB Polyclonal Antibody is a Rabbit antibody targeting CYBB. Anti-CYBB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-CYBB Polyclonal Antibody

Anti-CYBB Polyclonal Antibody

😃Good
Catalog No. TMAB-00514
Anti-CYBB Polyclonal Antibody is a Rabbit antibody targeting CYBB. Anti-CYBB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$374 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CYBB Polyclonal Antibody is a Rabbit antibody targeting CYBB. Anti-CYBB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-NOX2 Polyclonal Antibody, Unconjugated (TMAB-00514) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus.
2. Sample: LympHnode (Mouse) Lysate at 40 μg
Primary: Anti-NOX2 (TMAB-00514) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa
3. Sample: Spleen (Mouse) Lysate at 40 μg
Primary: Anti-NOX2 (TMAB-00514) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa
4. Sample:
Lane 1: Rat Bronchus tissue lysates
Lane 2: Rat Esophagus tissue lysates
Lane 3: Human HL60 cell lysates
Primary: Anti-NOX2 (TMAB-00514) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundNOX2/gp91phox is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. It also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of X-linked chronic granulomatous disease (X-CGD). X-CGD is characterized by the failure of activated phagocytes to generate superoxide. Patients suffer from life-threatening bacterial/fungal infections.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human NOX2
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cellular metabolic process,Cytotoxic Cells
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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