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Anti-CYBB Polyclonal Antibody
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Catalog No. TMAB-00514
Alias Superoxide-generating NADPH oxidase heavy chain subunit, p22 phagocyte B-cytochrome, NOX2, Neutrophil cytochrome b 91 kDa polypeptide, NADPH oxidase 2, Heme-binding membrane glycoprotein gp91phox, gp91-phox, gp91-1, Cytochrome b-245 heavy chain, Cytochrome b(558) subunit beta (Cytochrome b558 subunit beta), CYBB, CGD91-phox
Anti-CYBB Polyclonal Antibody is a Rabbit antibody targeting CYBB. Anti-CYBB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CYBB Polyclonal Antibody
Anti-CYBB Polyclonal Antibody
😃Good
Catalog No. TMAB-00514Alias Superoxide-generating NADPH oxidase heavy chain subunit, p22 phagocyte B-cytochrome, NOX2, Neutrophil cytochrome b 91 kDa polypeptide, NADPH oxidase 2, Heme-binding membrane glycoprotein gp91phox, gp91-phox, gp91-1, Cytochrome b-245 heavy chain, Cytochrome b(558) subunit beta (Cytochrome b558 subunit beta), CYBB, CGD91-phox
Anti-CYBB Polyclonal Antibody is a Rabbit antibody targeting CYBB. Anti-CYBB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CYBB Polyclonal Antibody is a Rabbit antibody targeting CYBB. Anti-CYBB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Superoxide-generating NADPH oxidase heavy chain subunit, p22 phagocyte B-cytochrome, NOX2, Neutrophil cytochrome b 91 kDa polypeptide, NADPH oxidase 2, Heme-binding membrane glycoprotein gp91phox, gp91-phox, gp91-1, Cytochrome b-245 heavy chain, Cytochrome b(558) subunit beta (Cytochrome b558 subunit beta), CYBB, CGD91-phox |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-NOX2 Polyclonal Antibody, Unconjugated (TMAB-00514) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus. 2. Sample: LympHnode (Mouse) Lysate at 40 μg Primary: Anti-NOX2 (TMAB-00514) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kDa Observed band size: 65 kDa 3. Sample: Spleen (Mouse) Lysate at 40 μg Primary: Anti-NOX2 (TMAB-00514) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kDa Observed band size: 65 kDa 4. Sample: Lane 1: Rat Bronchus tissue lysates Lane 2: Rat Esophagus tissue lysates Lane 3: Human HL60 cell lysates Primary: Anti-NOX2 (TMAB-00514) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kDa Observed band size: 65 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | NOX2/gp91phox is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. It also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of X-linked chronic granulomatous disease (X-CGD). X-CGD is characterized by the failure of activated phagocytes to generate superoxide. Patients suffer from life-threatening bacterial/fungal infections. |
| Immunogen | KLH conjugated synthetic peptide: human NOX2 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cellular metabolic process,Cytotoxic Cells |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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