Anti-CXCL16 Polyclonal Antibody is a Rabbit antibody targeting CXCL16. Anti-CXCL16 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse (predicted:Rat)

1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CXCL16 Polyclonal Antibody, Unconjugated (TMAB-00501) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Images kindly provided by Dr. Denny Cottle from the publication, Fetal inhibition of inflammation improves disease phenotypes in Harlequin Ichthyosis.Formalin-fixed and paraffin embedded mouse skin probed with Rabbit Anti-CXCL16 Polyclonal Antibody (TMAB-00501) at 1:50 for two hours at room temperature (green channel).
3. Blank control: A431. Primary Antibody (green line): Rabbit Anti-CXCL16 antibody (TMAB-00501)
Dilution: 3 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

IHC-P,IHC-Fr,IF,FCM

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 3ug/Test

Polyclonal

KLH conjugated synthetic peptide: the middle of human CXCL16

Human

Alpha Chemokines (CXC),Macrophage / Inflamm.