Anti-CX3CR1 Polyclonal Antibody is a Rabbit antibody targeting CX3CR1. Anti-CX3CR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Mouse,Rat (predicted:Human,Dog,Cow,Rabbit)

1. Sample:
Lane 1: Mouse Spinal cord tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Spinal cord tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Primary: Anti-CX3CR1 (TMAB-00494) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 47 kDa
2. Paraformaldehyde-fixed, paraffin embedded (rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CX3CR1) Polyclonal Antibody, Unconjugated (TMAB-00494) at 1:1000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-CX3CR1 antibody (TMAB-00494)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
4. Sample:
Heart (Mouse) Lysate at 40 μg
Heart (Rat) Lysate at 40 μg
Primary: Anti-CX3CR1 (TMAB-00494) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50/55 kDa
Observed band size: 53 kD

WB,IHC-P,IHC-Fr,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human CX3CR1

Human

Conjugates,Nociception,SARS Coronavirus,Host Virus Interaction,Delta Chemokine Receptors,HIV-related,Cell adhesion