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Anti-Cullin 4a Antibody (2X149)
😃Good
Catalog No. TMAC-01020
Alias MGC64071, MGC36573, Cullin4A, CUL4A_HUMAN, Cul4a protein, CUL-4A, Cul4a, CUL 4A, AW495282, 2810470J21Rik
Anti-Cullin 4a Antibody (2X149) is a Rabbit antibody targeting Cullin 4a. Anti-Cullin 4a Antibody (2X149) can be used in FCM,ICC,IHC,WB.
Anti-Cullin 4a Antibody (2X149)
Anti-Cullin 4a Antibody (2X149)
😃Good
Catalog No. TMAC-01020Alias MGC64071, MGC36573, Cullin4A, CUL4A_HUMAN, Cul4a protein, CUL-4A, Cul4a, CUL 4A, AW495282, 2810470J21Rik
Anti-Cullin 4a Antibody (2X149) is a Rabbit antibody targeting Cullin 4a. Anti-Cullin 4a Antibody (2X149) can be used in FCM,ICC,IHC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-Cullin 4a Antibody (2X149) is a Rabbit antibody targeting Cullin 4a. Anti-Cullin 4a Antibody (2X149) can be used in FCM,ICC,IHC,WB. |
| Synonyms | MGC64071, MGC36573, Cullin4A, CUL4A_HUMAN, Cul4a protein, CUL-4A, Cul4a, CUL 4A, AW495282, 2810470J21Rik |
| Ig Type | IgG |
| Clone | 2X149 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of Cullin 4a on Hela cell lysates using anti-Cullin 4a antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human cervix tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin. 7. ICC staining Cullin 4a in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining Cullin 4a in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of Hela cells with Cullin 4a antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 88 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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