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Anti-Cullin 1 Antibody (2P159)
😃Good
Catalog No. TMAC-01018
Alias MGC149835, MGC149834, Cullin-1, Cullin1, CUL1_HUMAN, CUL-1, CUL1, CUL 1
Anti-Cullin 1 Antibody (2P159) is a Rabbit antibody targeting Cullin 1. Anti-Cullin 1 Antibody (2P159) can be used in FCM,ICC/IF,IHC,IP,WB.
Anti-Cullin 1 Antibody (2P159)
Anti-Cullin 1 Antibody (2P159)
😃Good
Catalog No. TMAC-01018Alias MGC149835, MGC149834, Cullin-1, Cullin1, CUL1_HUMAN, CUL-1, CUL1, CUL 1
Anti-Cullin 1 Antibody (2P159) is a Rabbit antibody targeting Cullin 1. Anti-Cullin 1 Antibody (2P159) can be used in FCM,ICC/IF,IHC,IP,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $296 | 7-10 days | 7-10 days | |
| 100 μL | $496 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Cullin 1 Antibody (2P159) is a Rabbit antibody targeting Cullin 1. Anti-Cullin 1 Antibody (2P159) can be used in FCM,ICC/IF,IHC,IP,WB. |
| Synonyms | MGC149835, MGC149834, Cullin-1, Cullin1, CUL1_HUMAN, CUL-1, CUL1, CUL 1 |
| Ig Type | IgG |
| Clone | 2P159 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Cullin 1 on MCF-7 cell using anti-Cullin 1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cullin 1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Cullin 1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse oviduct tissue using anti-Cullin 1 antibody. Counter stained with hematoxylin. 5. Flow cytometric analysis of Hela cells with Cullin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).      |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC/IF: 1:10-50; IP: 1:10-50; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, also designated vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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