Anti-CTNNBIP1 Polyclonal Antibody is a Rabbit antibody targeting CTNNBIP1. Anti-CTNNBIP1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Chicken,Cow,Horse,Rabbit)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CTNNBIP1) Polyclonal Antibody, Unconjugated (TMAB-04760) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CTNNBIP1) Polyclonal Antibody, Unconjugated (TMAB-04760) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
3. Sample: U937 (Human) Cell Lysate at 30 μg
Primary: Anti-CTNNBIP1 (TMAB-04760) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 9 kD
Observed band size: 9 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CTNNBIP1

Human

CTNNBIP1

beta-catenin-interacting protein 1

Prevents the interaction between CTNNB1 and TCF family members, and acts as negative regulator of the Wnt signaling pathway.