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Anti-CT054 Polyclonal Antibody

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Catalog No. TMAB-04737

Anti-CT054 Polyclonal Antibody is a Rabbit antibody targeting CT054. Anti-CT054 Polyclonal Antibody can be used in WB,FCM.

Anti-CT054 Polyclonal Antibody

Anti-CT054 Polyclonal Antibody

Copy Product Info
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Catalog No. TMAB-04737
Anti-CT054 Polyclonal Antibody is a Rabbit antibody targeting CT054. Anti-CT054 Polyclonal Antibody can be used in WB,FCM.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2217-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5297-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-CT054 Polyclonal Antibody is a Rabbit antibody targeting CT054. Anti-CT054 Polyclonal Antibody can be used in WB,FCM.
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Dog,Cow)
Verified Activity
1. Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-CT054 antibody (TMAB-04737)
Dilution: 3 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
Testis (Rat) Lysate at 40 μg
Primary: Anti-CT054 (TMAB-04737) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Application
Recommended Dose
WB: 1:500-2000; FCM: 3μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Multi-pass membrane protein.
Tissue SpecificityPredominantly expressed in testis. Highly expressed in small intestine and prostate.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundCT054 is a Riboflavin transporter. Riboflavin transport is Na+-independent but moderately pH-sensitive. Activity is strongly inhibited by riboflavin analogs, such as lumiflavin, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), and to a lesser extent by amiloride.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CT054
Antigen Species
Human
Gene Name
SLC52A3
Gene ID
Protein Name
Solute carrier family 52, riboflavin transporter, member 3
Uniprot ID
Function
Riboflavin transporter. Riboflavin transport is Na(+)-independent but moderately pH-sensitive. Activity is strongly inhibited by riboflavin analogs, such as lumiflavin, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), and to a lesser extent by amiloride.
Chemical Properties
Molecular WeightTheoretical: 51 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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