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Anti-CSTF3 Polyclonal Antibody
Catalog No. TMAB-04735 Copy Product Info
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Anti-CSTF3 Polyclonal Antibody is a Rabbit antibody targeting CSTF3. Anti-CSTF3 Polyclonal Antibody can be used in WB.
Anti-CSTF3 Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-04735
Anti-CSTF3 Polyclonal Antibody is a Rabbit antibody targeting CSTF3. Anti-CSTF3 Polyclonal Antibody can be used in WB.
Anti-CSTF3 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
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| Description | Anti-CSTF3 Polyclonal Antibody is a Rabbit antibody targeting CSTF3. Anti-CSTF3 Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Human,Rat,Chicken,Dog,Cow,Rabbit) |
| Verified Activity | Sample: Thymus (Mouse) Lysate at 40 μg Primary: Anti-CSTF3 (TMAB-04735) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 83 kD Observed band size: 83 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nuclear. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. Heterotrimeric CstF recognizes GU- and U-rich sequences located downstream of the polyadenylation site on RNA. CstF-77 (cleavage stimulation factor, 77 kDa subunit), also known as CstF3, is one of the three subunits comprising CstF. It can exist as a homodimer and functions as the bridge, directly interacting with the other two CstF subunits, namely CstF-64 and CstF-50. CstF-77 is highly conserved among eukaryotes. It contains an Alpha-helical structure with 11 HAT (Half-a-TPR-containing) repeats and is essential for CstF assembly. In addition, CstF-77 is capable of interacting with CPSF1 and FCP1, other factors involved in polyadenylation. |
| Immunogen | KLH conjugated synthetic peptide: human CSTF3/CSTF77 |
| Antigen Species | Human |
| Gene Name | CSTF3 |
| Gene ID | |
| Protein Name | Cleavage stimulation factor subunit 3 |
| Uniprot ID | |
| Function | CSTF3 (cleavage stimulation factor subunit 3) is required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs and is part of the CSTF complex. The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF3 functions as a homodimer and interacts directly with CSTF1 and CSTF2. |
| Molecular Weight | Theoretical: 83 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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