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Anti-CSPG4 Antibody (2L981)

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Catalog No. TMAC-02839
Alias Melanoma-associated chondroitin sulfate proteoglycan, Melanoma chondroitin sulfate proteoglycan, MCSP, CSPG4, Chondroitin sulfate proteoglycan NG2, Chondroitin sulfate proteoglycan 4

Anti-CSPG4 Antibody (2L981) is a Rabbit antibody targeting CSPG4. Anti-CSPG4 Antibody (2L981) can be used in WB,IHC,FCM.

Anti-CSPG4 Antibody (2L981)

Anti-CSPG4 Antibody (2L981)

😃Good
Catalog No. TMAC-02839Alias Melanoma-associated chondroitin sulfate proteoglycan, Melanoma chondroitin sulfate proteoglycan, MCSP, CSPG4, Chondroitin sulfate proteoglycan NG2, Chondroitin sulfate proteoglycan 4
Anti-CSPG4 Antibody (2L981) is a Rabbit antibody targeting CSPG4. Anti-CSPG4 Antibody (2L981) can be used in WB,IHC,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2987-10 days
100 μL$4967-10 days
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Instruction Manual of Detection Antibody

Product Introduction

Bioactivity
Description
Anti-CSPG4 Antibody (2L981) is a Rabbit antibody targeting CSPG4. Anti-CSPG4 Antibody (2L981) can be used in WB,IHC,FCM.
SynonymsMelanoma-associated chondroitin sulfate proteoglycan, Melanoma chondroitin sulfate proteoglycan, MCSP, CSPG4, Chondroitin sulfate proteoglycan NG2, Chondroitin sulfate proteoglycan 4
Ig Type
IgG
Clone
2L981
Reactivity
Human
Verified Activity
1. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NG2 antibody. Counter stained with hematoxylin.
2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NG2 antibody. Counter stained with hematoxylin.
3. Flow cytometric analysis of SHG-44 cells with NG2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
WB
IHC
FCM
Recommended Dose
WB: 1:500-1000; IHC: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundNG2 (also known as melanoma-associated chondroitin sulfate proteoglycan 4, MCSP, MCSPG, MSK16 and MEL-CSPG) stabilizes cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. NG2 may facilitate primary melanoma progression by enhancing the activation of key signaling pathways important for tumor invasion and growth. Threonine 2256 phosphorylation of rat NG2 (Threonine 2252 phosphorylation of human NG2) leads to redistribution of NG2 on the surface of astrocytomas, polarization of the cell and a significant increase in cell motility. NG2 acts as a co-receptor for spreading and focal contact formation in association with α4 β1 integrin in malignant melanoma cells. NG2 is present on blood vessels throughout the rat embryo. Microvessels within the rat CNS express NG2 on endothelial cells, and outside the CNS, NG2 is present on smooth muscle cells. NG2 is a novel marker for epidermal stem cells that contributes to their patterned distribution by promoting stem cell clustering.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Q6UVK1
Chemical Properties
Molecular WeightTheoretical: 251 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

CSPG 4
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