Anti-CSNK2B/CK II beta Antibody (7S93) is a Rabbit antibody targeting CSNK2B/CK II beta. Anti-CSNK2B/CK II beta Antibody (7S93) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

7S93

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CSNK2B CK II beta) Monoclonal Antibody, Unconjugated (TMAB-04731) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CSNK2B CK II beta) Monoclonal Antibody, Unconjugated (TMAB-04731) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CSNK2B CK II beta) Monoclonal Antibody, Unconjugated (TMAB-04731) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CSNK2B CK II beta) Monoclonal Antibody, Unconjugated (TMAB-04731) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CSNK2B CK II beta) Monoclonal Antibody, Unconjugated (TMAB-04731) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CSNK2B CK II beta) Monoclonal Antibody, Unconjugated (TMAB-04731) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CSNK2B CK II beta) Monoclonal Antibody, Unconjugated (TMAB-04731) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CSNK2B CK II beta) Monoclonal Antibody, Unconjugated (TMAB-04731) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CSNK2B CK II beta) Monoclonal Antibody, Unconjugated (TMAB-04731) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CSNK2B CK II beta) Monoclonal Antibody, Unconjugated (TMAB-04731) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
11. Sample:
Lane 1: Mouse Cerebellum tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Testis tissue lysates
Lane 4: Rat Cerebellum tissue lysates
Lane 5: Rat Cerebrum tissue lysates
Lane 6: Rat Kidney tissue lysates
Lane 7: Rat Testis tissue lysates
Lane 8: Human Hela cell lysates
Lane 9: Human Jurkat cell lysates
Lane 10: Human K562 cell lysates
Lane 11: Human HepG2 cell lysates
Lane 12: Human MCF-7 cell lysates
Lane 13: Human SH-SY5Y cell lysates
Primary: Anti-CSNK2B/CK II beta (TMAB-04731) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa

WB: 1:500-2000; IHC-P: 1:50-100; IHC-Fr: 1:400-800; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human CSNK2B/CK II beta

Human

CSNK2B

Casein kinase II subunit beta

Participates in Wnt signaling. Plays a complex role in regulating the basal catalytic activity of the alpha subunit.