Anti-CRP Antibody (6D994) is a Mouse antibody targeting CRP. Anti-CRP Antibody (6D994) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

6D994

Human

1. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CRP(C11F2)) Monoclonal Antibody, Unconjugated (ascites of TMAB-04699 C11F2) at 1: 2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CRP(C11F2)) Monoclonal Antibody, Unconjugated (ascites of TMAB-04699 C11F2) at 1: 2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. Sample:
Lane 1: U2os (Human) Cell Lysate at 30 μg
Lane 2: Siha (Human) Cell Lysate at 30 μg
Primary:
Anti-CRP (TMAB-04699) at 1/1000 dilution
Anti-beta-Actin at 1/2000 dilution
Secondary:
IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 26 kD
4. Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CRP(C11F2)) Polyclonal Antibody, Unconjugated (TMAB-04699) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

Recombinant Protein: CRP protein

CRP

C-reactive protein

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.