Anti-Crotonyl-Histone H2B (Lys15/Lys16/Lys20) Antibody (9A793) is a Rabbit antibody targeting Crotonyl-Histone H2B (Lys15/Lys16/Lys20). Anti-Crotonyl-Histone H2B (Lys15/Lys16/Lys20) Antibody (9A793) can be used in WB.

IgG

9A793

Human

1. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1: 2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: (-): MCF-7, (+): MCF-7+sodium
butyrate (50mM, 24 h)+trichostain A (500 ng/ml, 4 hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 14 kDa
Observed MW: 14 kDa
2. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1: 2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: (-): HeLa, (+): HeLa+sodium butyrate (30 mM, 4 h)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 14 kDa
Observed MW: 14 kDa

WB: 1:500-2000

Monoclonal

KLH conjugated Synthesised acetylpeptide: human Histone H2B around the Crotonyl site of Lys15 / Lys16 / Lys20

Human

H2BC3

Histone H2B type 1-B

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.