Anti-CRNKL1 Polyclonal Antibody is a Rabbit antibody targeting CRNKL1. Anti-CRNKL1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Cow,Horse,Sheep)

1. Sample:
Siha (Human) Cell Lysate at 30 μg
HepG2 (Human) Cell Lysate at 30 μg
A549 (Human) Cell Lysate at 30 μg
Primary: Anti-CRNKL1 (TMAB-04692) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 100 kD
Observed band size: 98 kD
2. Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CRNKL1 Polyclonal Antibody, Unconjugated (TMAB-04692) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CRNKL1 Polyclonal Antibody, Unconjugated (TMAB-04692) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CRNKL1

Human

CRNKL1

Crooked neck-like protein 1

The crooked neck (crn) gene of Drosophila is essential for embryogenesis. It is thought to be involved in cell cycle progression and pre mRNA splicing.