Anti-CRMP3 Antibody (1H189) is a Rabbit antibody targeting CRMP3. Anti-CRMP3 Antibody (1H189) can be used in WB,FCM.

IgG

1H189

Human (predicted:Mouse,Rat)

1. The SH-SY5Y (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CRMP3 antibody (TMAB-04689, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 25 μg total protein per lane of various lysates (see on figure) probed with CRMP3 monoclonal antibody, unconjugated (TMAB-04689) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; FCM: 1:50-100

Monoclonal

A synthesized peptide: human DRP 4

Human

DPYSL4

Dihydropyrimidinase-related protein 4

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.